Hyphenation of ultra-high-performance liquid chromatography and ion mobility mass spectrometry for the analysis of sequence-defined oligomers with different functionalities and tacticity

In recent years, sequence-defined oligomers (SDOs) gained increasing interest due to their perfectly controlled molecular structure, thus providing defined properties. order tune the properties, different functionalities need be incorporated into and chain tacticity needs controlled. Beside synthesis of SDOs, suitable methods found analyze structure. this work, exhibiting an alternating or block-wise sequence side were analyzed using a hyphenation ultra-high-performance liquid chromatography electrospray ionization mass spectrometry enhanced by ion mobility separation (IMS). Moieties in chains varied according polarity bulkiness. Moreover, was varied. Drift times IMS cell corresponding collision cross section (CCS) values shown individual parameters allowing identification even case that SDO structures only differ functionalities. Thus, library CCS obtained as reference used for analysis complex mixtures SDOs.